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Products / SGE Analytical Science / GC Capillary columns

GC Capillary columns
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In this case, the hydrocarbon alkanes (light blue) are completely non-polar. They are retained on the phase only because the phase has sufficient non-polar character to interact with them. In the case of BPX90, it is so polar that it does not offer alkanes the opportunity for interaction. As a result, the alkanes tend to elute almost unretained. The alkanes show almost perfect orthogonality here. Retention on BPX5 versus no retention on BPX90 – they lie almost along the x-axis. We can now reason that if pure hydrocarbons (Van der Waals or non-polar interactions) give little or no BPX90 retention then retention of the remaining aromatics is due to purely p type interactions. When comparing GC phases, departures from the diagonal mark a significant change in the retention mechanism.

In conclusion, polar phases offer selectivity based on functionality rather than on Van der Waals interactions and are an ideal choice for the separation of analytes that were unresolved on non-polar or moderately polar phases.

The primary advantages of considering phase selectivity include:
• 2D GC – the choice of orthogonal chemistries for the 1st and 2nd dimensions.
• Fast GC – highly retained analytes on non-polar phases elute much earlier on polar phases.
• Ubiquitous FAMEs methods.
• Separation of unresolved analytes due to alternative functionality.
SGE hopes this information assists in your understanding of optimum GC capillary column phase selection for your application. Following is a summary of phase, plus other capillary column parameters such as internal diameter, capillary column length and film thickness, to assist with identification of the right SGE GC capillary column for your separation solution.
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