M/s. Gulf Analytical Supplies FZE have an established presence of nearly a decade in the middle east market and have a dominant presence in the field of separation science comprising mainly of chromatography with more than thousand columns supplied in the Middle East.  With the help of application support and technical support from our principals, we are the pioneers in the field of column chromatography.

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Products / SGE Analytical Science / HPLC Columns

HPLC Columns
The Role of Pore Size in Reversed Phase HPLC
SGE is excited to launch a new HPLC product line under the ProteCol™ brand.

Fundamental to the new ProteCol™ line of columns is the continued focus on inert column design that was first created with the ProteCol™ PEEKsil™ offering (polymer - sheathed fused silica tubing). SGE is excited to offer a new range of ultra pure reversed phase silicas in both the unique GLT™ (glass lined tubing) column format as well as a new PEEK™ lined stainless steel format. The benefit is the most comprehensive inert HPLC reversed phase column offering from the 150 micron ID PEEKsil™ format through to the new 4.6 mm ID PEEK™ lined stainless steel columns.

Why is Inert HPLC Column Design Important?
Non-specific interactions between the target analyte and the silica particles in the HPLC column are now well controlled with the availability of ultrapure silicas. Today most chromatographers expect that the silica sourced by manufacturers is of the highest purity and SGE confirms we have rigorously researched the quality of silicas using the standard reference material (SRM) provided by the National Institute of Standards & Technology. Testing of silicas using SRM 870 (NIST) identifies non-specific interactions associated with metal contamination as well as non-end capped silanols (see Figure 1).

What is often not considered is the role column hardware may play in non-specific interactions – the frit and internal column hardware can both influence the behavior of analytes with known metal chelating activity. Most pharmaceutically active compounds and natural products have the potential to interact with metals. Coordination between the metal ion and the analyte is facilitated by a lone electron pair on the analyte molecule.

If two electron donor groups (either oxygen or nitrogen) are located in a favorable position, a chelate can be formed and while the enthalpy of the complex formation for two monodentate ligands and a bidentate ligand is similar, the chelate is entropically favoured and leads to a stronger interaction. For this reason molecules like quinizarin,
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